Amino Acid Analysis
Proteins and peptides are large macromolecules consisting of covalently bonded amino acids. Proteins commonly exist as folded structures, while peptides are shorter linear polymers consisting of only a few amino acids. Amino acid analysis refers to the methodology used to determine the individual amino acids in a protein or peptide that may be part of—for example—a pharmaceutical preparation. Analysis of amino acid content can be used to support protein or peptide structure, evaluate fragmentation strategies, or detect atypical amino acids that may be present. It is necessary to hydrolyze the protein of interest, and the choice of procedures is key to accurate analysis as some sensitive amino acids may be destroyed during the hydrolysis. After hydrolysis, the hydrolyzing reagents are removed (typically by evaporation) and the hydrosylate is reconstituted in water or other compatible solvent. Because most free amino acids are poor chromophores, they cannot be directly detected at low concentrations using ultraviolet (UV), visible (vis), or fluorescence detection. Therefore the hydrolysate must first be derivatized with a chromophoric or fluorometric reagent before detection. This
amino acid analyzercan be performed either before chromatography (precolumn derivatization) or after chromatography (postcolumn derivatization). Both pre- and postcolumn derivatization methods are costly for both reagents and labor, add risk of toxic chemical exposure to personnel, and require hazardous waste removal. Electrochemical detection is a good alternative, that doesn't require derivatization after hydrolysis.
Main features:
This configuration is suitable for feed, food , chemical, pharmaceutical and other amino acid analysis, using pre-column derivatization method detachable 21 kinds or 18 kinds of amino acids. Can also be used as a binary HPLC .
Technical parameters:
(1) P98-Ⅰ type binary gradient infusion pump
Flow range : 0.01ml / min ~ 15ml / min
Flow rate accuracy :<3μl (0.01ml / min ~ 0.1ml / min), <1% (0.5ml / min ~ 9.99ml / min)
B liquid % Accuracy : = ± 0.5% ABS
Pressure range : 0.00MPa ~ 42MPa (420Kgf / cm2)
Microprocessor controlled proportional valve control encoder disk ; high accuracy.
Imported motor drive imports , subdivision twenty thousand steps, no mechanical noise ; to ensure accuracy.
Imported sealing systems , high pressure capability .
Single- piston reciprocating pump , a single one-way valve design solution without degassing .
Imported high pulsation damper , buffering capacity, so that the minimum ripple .
Multiple time program , do a gradient .
Automatic memory program , easy to bring up the methods.
Display: LCD screen display.
Display mode : the actual pressure , flow rate setting , the pressure lower limit .
Security Control : The pressure limit control , buzzer alarm, automatic shutdown.
Vertical chassis , touch panel.
(2) UV98-Ⅰ variable wavelength UV-Vis detector
Optical system : Imported grating monochromator design , imported photoelectric receiving device .
Light source: deuterium lamp Hamamatsu L6302
Wavelength range : 190 ~ 700nm
Wavelength accuracy : ± 1nm
Wavelength repeatability : ± 0.4nm
Noise : 2.5 × 10-5AU (pp) ( dynamic index , the mobile phase of methanol , 254nm, next column )
Drift : 1 × 10-3AU / hr ( dynamic index , the mobile phase of methanol , 254nm, next column )
Attenuation range : 0.002 ~ 2AU / mV 10 files
Detection cell volume : 4.5μl;
Detection cell voltage : 120 Kgf / cm2 ( patent pool structure design )
Minimum detectable amount : 2 × 10-8g / ml ( naphthalene / methanol solution )
Microprocessor-controlled , automatic scanning of stay ; wavelength repeatability.
LCD screen display, automatically find the wavelength , automatic zero adjustment.
Unique deuterium lamp control , striking voltage make deuterium lamp life up to 2500 hours.
Patented back pressure design, the bubble is not easy to enter the flow cell.
Vertical chassis , touch panel ; simple hydraulic connections .
Main features:
This configuration is suitable for feed, food , chemical, pharmaceutical and other amino acid analysis, using pre-column derivatization method detachable 21 kinds or 18 kinds of amino acids. Also available as two yuan HPLC
Dionex AAA-Direct
The Thermo Scientific* Dionex* AAA-Direct* system is a highly sensitive and direct-detection type of amino acid analyzer that requires no sample derivatization. The Dionex AAA-Direct instrument uses integrated pulsed amperometry (IPAD)—a type of electrochemical detection—to directly detect amino acids. The Dionex AAA-Direct also provides complete separation of all common amino acids using anion-exchange chromatography (Thermo Scientific Dionex AminoPac* PA10 column).
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